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KMID : 1161920140110020198
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Journal of Medicine and Life Science
2014 Volume.11 No. 2 p.198 ~ p.200
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Rapid purification of glutarthione S-transferase from Neodiplostomum seoulense
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Choi Min-Ho
Chung Young-Bae
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Abstract
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The authors partially purified glutathione S-transferase (GST) of Neodiplostomum seoulense monitoring its activity using 1- chloro-2,4-dinitrobenzene (CDNB). The enzyme was partially purified using glutathione?agarose affinity column chromatography. The purification fold and recovery rate were 9.7 and 50.4%, respectively. The molecular weight of the purified GST was estimated to be 28 kDa on 7.5-15% gradient SDS-PAGE. From this study, the authors firstly reported the presence of GST from N. seoulense and further studies such as inhibition test, subclass of GST as well as immunological studies would be required.
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KEYWORD
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glutathione S-transferase, Neodiplostomum seoulense, intestinal trematode
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